Table 3 Proteins with at least 1.2-fold expression change (P < 0.05, student t test) in serum of nonunion patients identified with the Ultraflex II MALDI mass spectrometer and Biotools v.3.l.software with an in-house hosted Mascot v2.2.2 server
From: Biomarkers of inflammation and innate immunity in atrophic nonunion fracture
No | Master no | Protein ID | Name | P value | Fold | pI | MW | Identification | Score Mascott |
|---|---|---|---|---|---|---|---|---|---|
1 | 2951 | APOA4_HUMAN | Apolipoprotein A–IV | 0.005 | −3.27 | 5.28 | 45371 | PMF | 78 |
2 | 2314 | APOE_HUMAN | Apolipoprotein E | 0.031 | 1.73 | 5.65 | 36246 | PMF | 93 |
3 | 2296 | APOE_HUMAN | Apolipoprotein E | 0.036 | 1.65 | 5.65 | 36246 | MS/MS | 34 |
4 | 2218 | APOE_HUMAN | Apolipoprotein E | 0.037 | 1.42 | 5.65 | 36246 | PMF | 86 |
5 | 2190 | APOE_HUMAN | Apolipoprotein E | 0.028 | 1.35 | 5.65 | 36246 | PMF | 133 |
6 | 1757 | ATX3_HUMAN | Ataxin-3 | 0.011 | −1.4 | 4.81 | 42097 | PMF | 57 |
7 | 961 | C4BPA_HUMAN | C4b-binding protein alpha chain | 0.0043 | 1.31 | 7.15 | 69042 | PMF and MS/MS | 72 and 44 |
8 | 956 | C4BPA_HUMAN | C4b-binding protein alpha chain | 0.034 | 1.29 | 7.15 | 69042 | PMF and MS/MS | 111 and 62 |
9 | 2200 | CLUS_HUMAN | Clusterin | 0.018 | 1.39 | 5.89 | 53031 | PMF and MS/MS | 70 and 63 |
10 | 2131 | CLUS_HUMAN | Clusterin | 0.02 | 1.22 | 5.89 | 53031 | PMF and MS/MS | 73and101 |
11 | 1946 | CLUS_HUMAN | Clusterin | 0.024 | 1.28 | 5.89 | 53031 | MS/MS | 17 |
12 | 3896 | CM016_HUMAN | Uncharacterized protein C13orf16 | 0.0079 | 1.37 | 4.99 | 16981 | PMF | 57 |
13 | 432 | co3_human | Complement C3 | 0.016 | 1.25 | 6.02 | 188569 | PMF | 119 |
14 | 444 | co6_human | Complement component C6 | 0.0029 | 1.44 | 6.39 | 108367 | PMF and MS/MS | 88 and 17 |
15 | 421 | co6_human | Complement component C6 | 0.032 | 1.27 | 6.37 | 108369 | PMF | 76 |
16 | 1883 | FHR1_HUMAN | Complement factor H-related protein 1 | 0.0012 | −1.58 | 7.38 | 38766 | PMF and MS/MS | 55 and 31 |
17 | 1874 | FHR1_HUMAN | Complement factor H-related protein 1 | 0.04 | 1.54 | 7.38 | 39766 | PMF | 85 |
18 | 974 | IGHM_HUM | lg mu chain C region | 0.018 | 1.22 | 6.35 | 49960 | PMF | 63 |
19 | 1766 | IGHM_HUMAN. MUCB_HUMAN. | lg mu chain C region. lg mu heavy chain disease protein | 0.021 | 1.75 | 6.35 | 49960 | MS/MS | 34 |
20 | 1765 | K1C10_HUMAN | Keratin. Type I cytoskeletal10 | 0.024 | −1.43 | 5.13 | 59020 | PMF | 57 |
21 | 1619 | PCOC1_HUMAN | Procollagen C-endopeptidase enhancer 1 | 0.05 | 1.25 | 7.41 | 48797 | PMF | 61 |
22 | 427 | RMND1_HUMAN | Required for meiotic nuclear division protein 1 homolog | 0.0023 | 1.34 | 8.88 | 51970 | PMF | 56 |
23 | 2528 | RN115_HUMAN | RING finger protein 115 | 0.043 | 1.21 | 5.39 | 34252 | PMF | 60 |
24 | 2660 | SAMP_HUMAN | Serum amyloid P-component | 0.017 | 1.25 | 6.1 | 25485 | PMF | 87 |