Biomarkers of inflammation and innate immunity in atrophic nonunion fracture

de Seny, Dominique; Cobraiville, Gaël; Leprince, Pierre; Fillet, Marianne; Collin, Charlotte; Mathieu, Myrielle; Hauzeur, Jean-Philippe; Gangji, Valérie; Malaise, Michel G.

doi:10.1186/s12967-016-1019-1

Journal of Translational Medicine

Table 2 Characterization of SELDI-TOF-MS biomarkers: P values were obtained according to the non-parametric Mann–Whitney U test (see also Additional file 4: Appendix Table 1)

From: Biomarkers of inflammation and innate immunity in atrophic nonunion fracture

m/z	MW	ID	Amino acid sequence	Regulation
m/z	MW	ID	Amino acid sequence	HV vs. NU	P value	Q value	Adj. P value
Hepcidin (P81172)
2191	2199	Hepcidin-20 (frag 65–84)	ICIFCCGCCHRSKCGMCCKT	Up	<0.001	0.007	0.023
2792	2797	Hepcidin-25 (frag 60–84)	DTHFPICIFCCGCCHRSKCGMCCKT	Up	<0.001	0.004	0.023
PACAP-related peptide (P18509)
4798	4800	PRP-48 (frag 82–129)	DVAHGILNEAYRKVLDQLSAGKHLQSLVARGVGGSLGGGAGDDAEPLS	Up	<0.001	0.021	0.037
Hemoglobin β subunit (P68871)
15866	15867	Hemoglobin β subunit (frag 2–147)	VHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESF GDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSE LHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVA GVANALAHKYH	Up	0.021	NS	NS
7931	15867	Hemoglobin β subunit (frag 2–147) [2H+]	VHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESF GDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSE LHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVA GVANALAHKYH	Up	<0.001	0.020	0.027
S100A8 (P05109) and S100A9 (P06702)
10844	10835	S100A8 (frag 1–93)	MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIR KKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE	Up	<0.001	0.008	0.0002
5419	10835	S100A8 (frag 1–93) [2H+]	MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIR KKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE	Up	<0.001	0.022
13269	13274	S100A9 (frag 1–114) [oxydized form]	MTCKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQ NFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASH EKMHEGDEGPGHHHKPGLGEGTP	Up	<0.001	0.004	0.0024
6346	12691	S100A9* (frag 5–114) [2H+] [Ser-actelyl]	S(ac)QLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQ NFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASH EKMHEGDEGPGHHHKPGLGEGTP	Up	<0.001	0.013
Inter-alpha-trypsin inhibitor H4 (ITIH4) (Q14624)
3157	3157	ITIH4 (isoform 1) frag 617–644	NVHSGSTFFKYYLQGAKIPKPEASFSPR	Down	<0.001	0.007	0.017
3179	3180	ITIH4 (isoform 1) frag 617–644 [aduct Na+]	NVHSGSTFFKYYLQGAKIPKPEASFSPR	Down	<0.001	0.007	0.017
3974	3972	ITIH4 (isoform 1) frag 650–687	QAGAAGSRMNFRPGVL SSRQLGLPGPPDVPDHAAYHPF	Down	<0.001	0.020
4282	4282	ITIH4 (isoform 2) frag 617–657	NVHSAGAAGSRMNFRPGVLSSRQLGLPGPPDVPDHAAYHPF	Down	<0.001	0.015
4299	4298	ITIH4 (isoform 2) frag 617–657 [Met.Ox]	NVHSAGAAGSRM(ox)NFRPGVLSSRQLGLPGPPDVPDHAAYHPF	Down	<0.001	0.015
Complement C3 alpha chain (P01024)
8132	8132	C3a (frag 672–739)	SVQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLG EACKKVFLDCCNYITELRRQHA	Down	<0.001	0.022	NS
Apolipoprotein A–I (P02647)
28106	28061	ApoA1 (frag 25–267)	DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKL	Down	<0.001	0.029	NS
28099			LDNWDSVTST FSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEE VKAKVQPYLDDFQK KWQEEMELYRQKVEPLRAELQEGARQKLHEL QEKLSPLGEEMRDRARAHVDALRTHLAPY SDELRQRLAARLEALKEN GGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNT	Down	<0.001	0.05

Associated Q values were calculated using FDR (1000 permutations) for multiple testing. Adjusted P value for age, gender, smoking status, consumption of alcohol and the presence of pulmonary diseases were performed by multivariate analysis on the SAS software version 9.4
m/z mass/charge ratio of the peak detected by SELDI-TOF-MS, MW molecular weight of the related protein or peptide, ID identification of the related protein or peptide, NS not significant

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ISSN: 1479-5876

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