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Table 2 Primers and probes employed in the multiplex quantitative real-time PCR

From: A validated cellular biobank for β-thalassemia

Primer

Sequence

Forward primer α-globin

5′-CGACAAGACCAACGTCAAGG-3′

Reverse primer α-globin

5′-GGTCTTGGTGGTGGGGAAG-3′

α-globin probe

5′-HEX-ACATCCTCTCCAGGGCCTCCG-BHQ-3′

Forward primer β-globin

5′-GGGCACCTTTGCCACAC-3′

Reverse primer β-globin

5′-GGTGAATTCTTTGCCAAAGTGAT-3′

β-globin probe

5′-Texas Red-ACGTTGCCCAGGAGCCTGAAG-BHQ-3′

Forward primer γ-globin

5′-TGACAAGCTGCATGTGGATC-3′

Reverse primer γ-globin

5′-TTCTTTGCCGAAATGGATTGC-3′

γ-globin probe

5′-FAM-TCACCAGCACATTTCCCAGGAGC-BHQ-3′

Forward primer RPL13A

5′-GGCAATTTCTACAGAAACAAGTTG-3′

Reverse primer RPL13A

5′-GTTTTGTGGGGCAGCATACC-3′

RPL13A probe

5′-CY5-CGCACGGTCCGCCAGAAGAT-BHQ-3′

  1. The 5′ and 3′ chromogenic molecules are underlined in the sequence