Table 1 Primers used in the polymerase chain reactions and in the sequencing reactions for β-globin gene
Primer | Sequence (5′–3′) | Tm (°C) |
|---|---|---|
Forward primer BGF | 5′-GTGCCAGAAGAGCCAAGGACAGG-3′ | 72.1 |
Forward primer T12F | 5′-AGACCTCACCCTGTGGAGCC-3′ | 67.9 |
Forward primer T3F | 5′-ACAATCCAGCTACCATTCTGCTTT-3′ | 65.7 |
Forward primer BG6F | 5′-CGCTTTCTTGCTGTCCAATTTC-3′ | 66.7 |
Forward primer BG5SF | 5′-GCCTGGCTCACCTGGACA-3′ | 66.7 |
Reverse primer BG4 | 5′-TCAGGAGTGGACAGATCCCC-3′ | 66.5 |
Reverse primer T12R | 5′-AGTTCTCAGGATCCACGTGCA-3′ | 67.1 |
Reverse primer BGR | 5′-CACTGACCTCCCACATTCCCTTTT-3′ | 69.8 |
Reverse primer BGi2R | 5-‘GTTGCCCAGGAGCTGTGG-3′ | 67.1 |