Fig. 4

a Erythroid differentiation (% of benzidine-positive, haemoglobin containing cells) evaluated at different days (as indicated) of the cell cultures from different patients (each color represent one subject) (*P < 0.05). b, c HPLC analysis of the ErPCs cell cultures show representative examples of lysates containing moderate (b) and high (c) HbF endogenous levels. d Correlation between HbF levels determined using the two different cell culture protocols, the Fibach’s method (“Two-phases procedure”) and the protocol based on biobanked cells described in the present manuscript