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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Inhibition of complement improves graft outcome in a pig model of kidney autotransplantation

Fig. 5

Immunohistochemical staining on 3 months post-transplant kidney samples. Pigs were euthanized after 3 months follow up and kidney biopsies were collected and processed for immunohistochemical analysis. a Vimentin staining, specific for EMT. Left and middle representative staining for each group. Magnification: ×100. Right quantification of the area covered by the staining per high powered field (×100). b α-SMA staining, specific for EMT. Left and middle representative staining for each group. Magnification: ×100. Right quantification of the area covered by the staining per high powered field (×100). c Western blot analysis of TGF-β signaling; top: representative blots for each marker; bottom: quantification of the signal normalized to β-actin. Shown are mean ± SD, n = 7. Statistics: *p < 0.05. Vimentin staining data were normally distributed but did not show equality of variance on the Variance-Ratio Equal-Variance Test, hence non parametric analysis was performed. α-SMA staining data were normally distributed and had equal variance, hence parametric analysis was performed. TGF-β protein level values were normally distributed but did not show equality of variance on the Variance-Ratio Equal-Variance Test, hence non parametric analysis was performed. P-Smad3/Smad3 ratio values were normally distributed and had equal variance, hence parametric analysis was performed

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