Fig. 6

Celecoxib prevents lysosomal function. a KBM5 and KBM5-T315I cells were treated with 20 μM CQ or 40 μM celecoxib for 24 h. Hoechst 33342 staining and LysoTracker (Cat. No. L7526) probe labelling assay was used to detect autophagosomes amount and the fluorescence intensity was showed by confocal microscopy. b The cells were treated as above, then LysoSensor (Cat. No. L7535) probes labelling assay were conducted to determinate the pH value inside lysosome. The results shown are representative of three independent experiments