Fig. 2

Bromocriptine treatment affected cell viability of primary AML samples, sparing healthy blood cells. AML primary patient samples were treated for 24 (a) or 72 (b) h with vehicle control or 10 µM bromocriptine. Cell viability was measured by flow cytometry. Primitive population corresponds to the CD34⁺CD38⁻ AML fraction. Live cells refer to control are represented, each symbol type represents an individual AML patient and each symbol corresponds to an independent experimental point. c Cell viability 72 h after 10 µM bromocriptine treatment was analyzed inside the blast and non-blast population (according to the SSC-CD45 profile). d Vehicle control- or bromocriptine-treated primary AML samples were assayed for the expression of CD11b. The relative frequency of CD11b-positive cells refer to control is represented. e Primary AML samples were treated for 18 h and cultivated in methylcellulose. 14 days after, colonies were screened based on cellularity and morphology criteria. Bars represent mean value of at least biological triplicates. Error bars correspond to SEM. CFU-B: CFU-Blasts. ***p < 0.005; ****p < 0.001