Fig. 5

T cell memory subset analysis in human MM and Vk*MYC mouse models. a Human PBMC CD4⁺/CD8⁺ T cell memory subset analysis. (i–ii) Box and whisker plots showing T cell memory subsets (i) CD4⁺ T cells (ii) CD8⁺ T cells. FACS plots showing representative staining are shown to the left: T cell subsets are expressed as %CD3ε⁺CD4⁺ or %CD3ε⁺CD8⁺ T cells and are defined as naïve/T_N (CD45RA⁺CCR7⁺), central memory/T_CM (CD45RA⁻CCR7⁺), effector memory/T_EM (CD45RA⁻CCR7⁻), and terminally differentiated effector memory T cells/T_EMRA (CD45RA⁺CCR7⁻). Clear boxes ARCBS donors, light grey boxes NDMM, dark grey boxes RRMM. b T cell memory subset matched pair analysis in PBMC and BMMC from NDMM patients (as described in a). (i) CD4⁺ T cells (ii) CD8⁺ T cells. c Murine BMMC T cell memory subset analysis in transgenic Vk*MYC. (i) FACS plots showing representative staining: T cell subsets are expressed as %TCRβ⁺CD8⁺ or %TCRβ⁺CD8⁻ (CD4⁺) and are defined as naïve/T_N (CD44⁻CD62L⁺), central memory/T_CM (CD44⁺CD62L⁻) and effector memory/T_EM (CD44⁺CD62L⁻). (ii–iii) Box and whisker plots comparing WT to diseased transgenic Vk*MYC. (ii) CD4⁺ T cells (iii) CD8⁺ T cells. Closed circles represent a-m WT, open circles represent diseased mice (which are subdivided into low, intermediate (int.) or high-grade disease as described in Fig. 2a). d Dot plot showing correlation between %CD8⁺ T_EM and %IFNγ⁺CD8⁺ T cells in transgenic Vk*MYC mice. e Murine BMMC T cell memory subset analysis in a diseased cohort of transplant Vk#4929 (flow cytometry as described for c). Clear boxes/closed circles represent a-m WT, dark grey boxes/open circles represent diseased mice