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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: The GLP-1 receptor agonists exenatide and liraglutide activate Glucose transport by an AMPK-dependent mechanism

Fig. 6

EXE induces 2DG-uptake via AMPKα activation in L6 myotubes. L6 myotubes were transfected with specific AMPK siRNAs. After transfection, the cells were stimulated with 100 nmol/l EXE or 100 nmol/l INS. Proteins were then extracted and probed with AMPKα (a) and phospho-specific AMPKα T172 (b) antibodies. To normalize the blots for protein levels, the blots were stripped and re-probed with anti-tubulin (a, lower panel) and AMPKα antibodies (b, lower panel). 2DG-uptake was measured in cells after stimulation with INS or EXE (c). Data are represented as fold increase over control ± SD of three independent experiments (*p < 0.001, **p < 0.01 vs basal). 2DG-uptake was also measured in cells transfected with the AMPK siRNA 2 or control SiNR, before and after stimulation with EXE. Data are shown as fold increase over control ± SD of three independent experiments (d) (**p < 0.01 vs basal)

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