Fig. 4

Time-course and dose–response experiments of EXE effects on AMPK and ACC phosphorylation in L6 cells. Phosphorylated and total (a, b, c, upper panels) ACC S78/80 (a) and AMPKa T172 (b) protein levels were measured in cells exposed to EXE for increasing time periods. For the dose response curve (c) cells were incubated for 20 min with 0, 1, 10 and 100 nmol/l EXE. Thr172 phosphorylation and total levels of AMPK were shown. Each bar represents the mean ± SD of three independent experiments. (*P < 0.01 and **P < 0.02 vs. control)