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Table 2 Chemical and physical parameters analyzed during the purification of the HPV16 His6-E6 protein under native conditions

From: Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy

Protocol Temperature (°C) Dithiothreitol (µM) pH Imidazole (mM) Detergenta
Lysis buffer Wash buffer Elution buffer  
A Room temperature 8 10 20 250
B 4 100 8 10 25 250
C 4 100 8 20 50 250
D 4 100 7.5 20 70 300
E 4 100 7.5 20 70 300 0.02 % lauryl-β-D-maltoside
0.02 % Tween-20
0.02 % glycine
0.02 % betaine
0.1 M arginine
F 4 100 7.5 20 70 300 0.02 % betaine
  1. aThe detergents listed were tested individually