Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy

Table 2 Chemical and physical parameters analyzed during the purification of the HPV16 His₆-E6 protein under native conditions

Protocol	Temperature (°C)	Dithiothreitol (µM)	pH	Imidazole (mM)			Detergent^a
Protocol	Temperature (°C)	Dithiothreitol (µM)	pH	Lysis buffer	Wash buffer	Elution buffer
A	Room temperature	–	8	10	20	250	–
B	4	100	8	10	25	250	–
C	4	100	8	20	50	250	–
D	4	100	7.5	20	70	300	–
E	4	100	7.5	20	70	300	0.02 % lauryl-β-D-maltoside 0.02 % Tween-20 0.02 % glycine 0.02 % betaine 0.1 M arginine
F	4	100	7.5	20	70	300	0.02 % betaine

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