E. coli strain
|
Combinationa according to chaperoneb (plasmid)
|
---|
|
A (pG-KJE8)
|
B (pGro7)
|
C (pKJE7)
|
D (pG-Tf2)
|
E (pTf16)
|
---|
M15 [pREP4]
|
–
|
++
|
++++
|
–
|
++
|
BL21 (DE3)
|
–
|
++
|
+
|
–
|
+
|
JM109
|
+
|
+++
|
+
|
–
|
+++++
|
- ± Qualitative estimation of yield
-
aAll combinations included the pQE30 HPV16 His6-E6 plasmid
-
bChaperone A carried the dnaK-dnaJ-grpE groES-groEL genes, B the groES-groEL genes, C the dnaK-dnaJ-grpE genes, D the groES-groEL-tig genes, E the tig gene