Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy

Table 1 Different combinations of molecular chaperones and E. coli strains tested for HPV His₆-E6 expression

E. coli strain	Combination^a according to chaperone^b (plasmid)
	A (pG-KJE8)	B (pGro7)	C (pKJE7)	D (pG-Tf2)	E (pTf16)
M15 [pREP4]	–	++	++++	–	++
BL21 (DE3)	–	++	+	–	+
JM109	+	+++	+	–	+++++

± Qualitative estimation of yield
^aAll combinations included the pQE30 HPV16 His₆-E6 plasmid
^bChaperone A carried the dnaK-dnaJ-grpE groES-groEL genes, B the groES-groEL genes, C the dnaK-dnaJ-grpE genes, D the groES-groEL-tig genes, E the tig gene

ISSN: 1479-5876