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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Production of functional, stable, unmutated recombinant human papillomavirus E6 oncoprotein: implications for HPV-tumor diagnosis and therapy

Fig. 1

Effects of detergents on HPV16 His6-E6 solubility. Representative immunoblots of the soluble HPV16 His6-E6 protein before dialysis (lanes 1), after dialysis (lanes 2), and after high-speed centrifugation (lanes 3), in the absence of detergent (a) and with addition of lauryl-β-D-maltoside (a), glycine (b), Tween-20 (b), arginine (c) and betaine (c). C+ , HPV16 His6-E6 protein purified under denaturing conditions. (d) Representative SDS-PAGE of the HPV16 His6-E6 protein purified through the optimized protocol

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