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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: In vivo monitoring of lung inflammation in CFTR-deficient mice

Fig. 4

Representative in vivo images of WT and CF mice transiently transgenized with bIL-8-Luc intratracheally challenged with VR1 culture supernatant (10X/mouse), and monitoring of bIL-8 activation by BLI. a, d: WT and CF mice after intratracheal instillation with TSB (control). Panel B, E: WT and CF mice after intratracheal instillation with VR1Sn and monitoring of IL-8 activation at 4 and 24 h by BLI. c, f: ex vivo images of IL-8 activation in lungs excised from WT and CF mice at 24 h. g: Time-course of IL-8-luc activation in WT and CF mice after challenge with intratracheal VR1Sn. Results are reported as FOI over baseline as mean ± SEM, n = 6 for each group. Panel H: Cellular infiltration into the lung of WT and CF mice after intratracheal instillation with VR1Sn. VR1Sn-induced neutrophils and white blood cells (WBC) recruitment in the airways at 24 h. Three mice were used for every time point and the experiment was replicated three times. Values are expressed as mean ± SEM of the three different experiments. Statistical differences were tested by one-way ANOVA followed by Dunnett’s t post hoc test for group comparisons. *p < 0.05 and **p < 0.01 WT and CF TSB vs WT and CF VR1 group. (in) : Representative micro-photographs Giemsa staining of lung of WT and CF mice after intratracheal instillation with TSB(I, L), and VR1Sn (M, N) at 24 h. Magnification 40×

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