Fig. 4

Representative blots (top) and quantitative protein expression (bottom) of total and phosphorylated eNOS (p-eNOS) at Ser1177 (A); dimerized eNOS (B); total nNOS (C) and catalase (D) in aorta from sedentary (S) and exercise-trained (Ex) wild-type (WT) and LDLr knockout mice (LDLr^−/−). Proteins expression were normalized to α-actin content in each sample, and protein expression of p-eNOS were normalized to total eNOS expression. eNOS dimerization was expressed as a ratio of dimer:monomer band intensity. The results were expressed as the percentage of the protein expression values obtained in WT S group. Data are mean ± SEM. Two-way ANOVA: * p < 0.05 vs. WT S; ^$ p < 0.05 vs. WT Ex; ^# p < 0.05 vs. LDLr^−/− S. Numbers into the bars represent N of animals used in each group