Fig. 3
Representative fluorographs (A, left panel) and quantitative analysis (A, bar graphic right) of NO production evaluated by DAF-2A fluorescence in transverse sections of thoracic aorta. DAF-2A fluorescence is expressed as the percentage of intensity per vessel area obtained in WT S group (bar scale = 100 μm). The bar graph (B) represents the fluorescence curve slope to Amplex Red estimating the production of H2O2 per minute in aortic rings from sedentary (S) and exercise-trained (Ex) wild-type (WT) and LDLr knockout mice (LDLr−/−). Data are mean ± SEM. Two-way ANOVA: * p < 0.05 vs. WT S; $ p < 0.05 vs. WT Ex; # p < 0.05 vs. LDLr−/− S. Numbers into the bars represent N of animals used in each group