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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Invariant asymmetry renews the lymphatic vasculature during homeostasis

Fig. 4

Live imaging methodology for capturing the same fields of interest overtime within the Lyve1CreERT2tdT pinna containing single tdT+ clones and small populations. A single 0.25 mg 4-OHT dose was administered to 4 Lyve1CreERT2tdT mice to induce tdT fluorescence stochastically in individual cells. Using low power brightfield microscopy, regions of interest within the pinna were identified based on the morphologic features of large blood vessels (ac). An asterisk shows a blood vessel branch point as an example. Without adjusting the position of the pinna or the focus, the Texas Red filter was used to detect the endogenous tdT fluorescence (df). The magnified fields show examples of single tdT+ clones (arrow) and a tdT+ small population of 3 cells (arrowhead) (gi). The tdT+ cells were visualized 20 (g), 25 (h), and 44 (i) days following labeling. This methodology was used to identify 110 tdT+ clones and 80 tdT+ small populations that were tracked over time. The size standard is 100 µm

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