Fig. 3
From: Invariant asymmetry renews the lymphatic vasculature during homeostasis
Expression of tdT fluorescence in Lyve1CreERT2tdT pinna following low dose 4-OHT administration. Three weeks after 0.25 mg 4-OHT was administered to 6 Lyve1CreERT2tdT mice, the pinnas were harvested and labeled with antibodies to LYVE-1. Maximum intensity projection images obtained using confocal microscopy were used to determined the targeting specificity of the transgene. 2 images from each mouse pinna were analyzed. Using images similar to the image shown in a, tdT+ cells were quantified. Using images similar to that shown in b, it was determined whether the tdT+ cells were LYVE-1+ or LYVE-1– and whether these cells were a constituent of a lymphatic vessel. Most of the tdT+ cells were LYVE-1+ and were constituents of lymphatic vessels (93 %). Some of the tdT+ cells were LYVE-1+ or LYVE-1− and physically separated from a lymphatic vessel (7 %) (b, c). The histogram is data pooled from fields obtained from 6 similarly treated Lyve1CreERT2tdT mice. The size standards are 50 µm