Skip to main content
Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Invariant asymmetry renews the lymphatic vasculature during homeostasis

Fig. 2

Little remodeling was detected in the lymphatic vessel network in the Lyve1CreERT2tdT pinna. The experimental strategy is illustrated in a. The vertical lines represent points in time when live imaging was performed. 4-OHT was administered to Lyve1CreERT2tdT mice to label LECs within the pinna. Live imaging epifluorescent microscopy of 2 sedated Lyve1CreERT2tdT mice was performed, first every 3 or 4 days, then with increasing intervals, at 3 low magnification fields within the right pinna to directly visualize the tdT+ lymphatic vessels. The morphology of the tdT+ lymphatic vessel network within the microenvironment was detectable and relatively stable over the course of 323 days post-labeling (panel B). The overall morphology of many of the lymphatic vessels was similar over the course of the study (b-inset). At the conclusion of this study, the pinnas were labeled with antibodies to LYVE-1 and evaluated using immunofluorescent microscopy techniques. Low power epifluorescent microscopy showed that most of the tdT+ cells were LYVE-1+ LECs comprising lymphatic vessels (c–e). The results are representative of 2 different Lyve1Cre-ERT2tdT mice. The size standards are 100 µm

Back to article page