Fig. 3
GRIM19 regulates T helper cell responses. a Recipients (n = 6 B/c mice per group) were intravenously injected with 5 × 106 donor (WT or GRIM19 Tg) bone marrow cells and 1 × 107 WT or GRIM19 Tg splenocytes after lethal irradiation. Intracellular cytokines were detected in CD4+ T cells. Fourteen days after BMT, splenocytes were isolated and the expression levels of IL-4, IL-17, and IFN-γ were determined by flow cytometry. b The mRNA levels of Il-17, Il-4, Treg, and Ifn-γ were analyzed by quantitative PCR. **p < 0.01. c Fourteen days after BMT, splenocytes were isolated and stained with anti-CD4 followed by reacting with anti-IL-17, anti-IL-4, and anti-IFN-γ antibodies. The expression patterns were visualized by confocal microscopy. d Expression levels of intracellular signaling molecules p-STAT3 (Tyr705) and p-STAT3 (Ser727) in splenic CD4+ T cells were determined by confocal microscopy on day 14 after allogeneic BMT. Confocal microscopy images were obtained for each mouse (n = 6 per group) and representative images are shown. Bars represent the mean ± SD from six mice per group. *p < 0.05, **p < 0.01. Data are representative of at least three independent experiments