Fig. 3

LPS-induced BEAS-2B cells damage was attenuated by enanthol, but amplified by RA. a “Parachute” dye-coupling assay was used to determine effects of inhibitor, gap26 (300 μM, for 1 h) and enhancer RA, (10 μM, for 24 h) on GJ composed of Cx43. b, c Effects of gap26 (300 μM, for 1 h) and RA (10 μM, for 24 h) on cell growth and LDH release exposed to LPS (5 μg/ml, for 24 h), n = 5, *P < 0.05 vs control; ^#P < 0.05 vs LPS. d–g Effects of gap26 (300 μM, for 1 h) and RA (10 μM, for 24 h) on TNF-α, IL-1β, IL-6 and IL-8 exposed to LPS (5 μg/ml, for 24 h). Vehicle controls of gap26 and RA were DMSO, n = 5, *P < 0.05 vs control; ^#P < 0.05 vs LPS