Fig. 5From: Detection of tumor-associated cells in cryopreserved peripheral blood mononuclear cell samples for retrospective analysisDetection of Circulating tumor cells (CTCs) in the matched fresh and frozen renal cell carcinoma samples (RCC patient 2). A CTC is defined as a nucleated cell positive for both CKs (green) and vimentin (red) but negative for CD45 (magenta). a Fresh peripheral blood mononuclear cell sample from a selected patient with renal cell carcinoma, without cryopreservation. b Frozen peripheral blood mononuclear cell sample from the same patient with renal cell carcinoma, with cryopreservation for 7 days. The top row shows a cluster of CTCs with one cell in mitosisBack to article page