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Table 1 Primers used in plasmid construction

From: HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells

Name

Primer sequence

pMiR-1269b-luc sense

5′-GACAACTCGAGTGCCCTCACCTTGCCC-3′

pMiR-1269b-luc anti-sense

5′-GACCGAATTCAAATGTCAAAGAACCTGG-3′

pMiR-1269b-luc-mut sense

5′-GGCCAGGTACCTGCCCTCACCTTGCCC-3′

pMiR-1269b-luc-mut anti-sense

5′-AGCGAATTCCAAACCAAGGAGCAAGTCCG-3′

pre-miR-1269b sense

5′-CGCGGATCCACTGAGTTTCGGGTTCTG-3′

pre-miR-1269b anti-sense

5′ -CGGAATTCGTTGGCTCACATAATCACA-3′

ASO-miR-1269b

5′-CCAGUAGCAUGGCUCAGUCCAG-3′

ASO-NC

5′-CAGUACUUUUGUGUAGUACAA-3′

CDC40 sense

5′-GAAGAATTCATGTCGGCTGCGATTGCAGCTCTGGC-3′

CDC40 anti-sense

5′-GAAGACTCGAGCGATCCCACAATTTAATGAGACCATCCCAAC-3′

CDC40-shR-top

5′-GATCCGTGGTTGGGATGGTCTCATTACTCGAGTAATGAGACCATCCCAACCACTTTTTGA-3′

CDC40-shR-bottom

5′-AGCTTCAAAAAGTGGTTGGGATGGTCTCATTACTCGAGTAATGAGACCATCCCAACCACG-3′

CDC40-3′UTR sense

5′-GATCCAAGTACGAGGGGTAGAGTCCAAGATGTCAACTTGTGGTACCG-3′

CDC40-3′UTR anti-sense

5′-AATTCGGTACCACAAGTTGACATCTTGGACTCTACCCCTCGTACTTG-3′

CDC40-3′UTRmut sense

5′-GATCCAAGTACGAGGGGTAGACTGGAAGATGTCAACTTGTGGTACCG-3′

CDC40-3′UTRmut anti-sense

5′-AATTCGGTACCACAAGTTGACATCTTCCAGTCTACCCCTCGTACTTG-3′