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Table 1 Primers used in plasmid construction

From: HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells

Name Primer sequence
pMiR-1269b-luc sense 5′-GACAACTCGAGTGCCCTCACCTTGCCC-3′
pMiR-1269b-luc anti-sense 5′-GACCGAATTCAAATGTCAAAGAACCTGG-3′
pMiR-1269b-luc-mut sense 5′-GGCCAGGTACCTGCCCTCACCTTGCCC-3′
pMiR-1269b-luc-mut anti-sense 5′-AGCGAATTCCAAACCAAGGAGCAAGTCCG-3′
pre-miR-1269b sense 5′-CGCGGATCCACTGAGTTTCGGGTTCTG-3′
pre-miR-1269b anti-sense 5′ -CGGAATTCGTTGGCTCACATAATCACA-3′
ASO-miR-1269b 5′-CCAGUAGCAUGGCUCAGUCCAG-3′
ASO-NC 5′-CAGUACUUUUGUGUAGUACAA-3′
CDC40 sense 5′-GAAGAATTCATGTCGGCTGCGATTGCAGCTCTGGC-3′
CDC40 anti-sense 5′-GAAGACTCGAGCGATCCCACAATTTAATGAGACCATCCCAAC-3′
CDC40-shR-top 5′-GATCCGTGGTTGGGATGGTCTCATTACTCGAGTAATGAGACCATCCCAACCACTTTTTGA-3′
CDC40-shR-bottom 5′-AGCTTCAAAAAGTGGTTGGGATGGTCTCATTACTCGAGTAATGAGACCATCCCAACCACG-3′
CDC40-3′UTR sense 5′-GATCCAAGTACGAGGGGTAGAGTCCAAGATGTCAACTTGTGGTACCG-3′
CDC40-3′UTR anti-sense 5′-AATTCGGTACCACAAGTTGACATCTTGGACTCTACCCCTCGTACTTG-3′
CDC40-3′UTRmut sense 5′-GATCCAAGTACGAGGGGTAGACTGGAAGATGTCAACTTGTGGTACCG-3′
CDC40-3′UTRmut anti-sense 5′-AATTCGGTACCACAAGTTGACATCTTCCAGTCTACCCCTCGTACTTG-3′