Fig. 4

miR-1269b directly targets the 3′UTR of CDC40. a The predicted binding sites for miR-1269b in the 3′UTR of CDC40 and the induced mutations in the binding sites are shown. b qRT–PCR (left) and representative Western blot analysis (right) of CDC40 levels in HepG2 and SMMC-7721 cells transfected with the control vector or ASO-nc vs miR-1269b or ASO-miR1269b-transduced cells. c Luciferase reporter assays were performed in HepG2 and SMMC-7721 cell lines that were co-transfected with miR-1269b in the presence of CDC40 3′UTR (top). In the controls, the mutated 3′UTR sequences were included (bottom). Columns, mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001