Fig. 3

High expression levels of miR-1269b promoted the proliferation and migration of HepG2 and SMMC-7721 cells in vitro. a HepG2 and SMMC-7721 cells were transfected with pcDNA3/pri-miR-1269b, ASOmiR-1269b or a negative control vector in 24-well plates and then seeded in 12-well plates. The number of colonies was counted at 2 weeks after seeding. b Cell cycle progression was analyzed in HepG2 and SMMC-7721 cells using flow cytometry. The chart shows the population of cells that were in G1-, S- and G2/M-phase. c Transwell migration assays were performed to detect the migratory capacity of HepG2 and SMMC-7721 cells transfected with the same vectors. d The influence of miR-1269b on the protein levels of the EMT-associated molecules E-cadherin and vimentin were measured using western blot analysis. All error bars indicate the means ± SDs. All experiments were repeated at least three times. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001