Fig. 1

Comb1 and UN3 enhance wound-healing re-epithelialization and angiogenesis in diabetic swine. (a–d) Representative trichrome staining of wounds treated with saline for 3 days (a) or 7 days (c) and Comb1 + UN3 (b, d). Scale bar (black) denotes 1 mm in all photomicrographs of trichrome-stained tissues, and the yellow line denotes the linear distance traversed by migrating keratinocytes. Representative HSPG (green) and CD31 (red) co-staining for each treatment condition and time point are displayed as insets in upper right of each panel; scale bar (white) denotes 100 μm, and the dashed white line in the insets of (a) and (b) denotes the lateral border between injured and non-injured tissue. Data in (e) are expressed as mean wound re-epithelialization, width, granulation tissue area, and microvascular density normalized to saline-treated controls on each day, ±SEM, *p ≤ 0.05, **p ≤ 0.01. For re-epithelialization and wound width measurements: Saline day 3, n = 9; Comb1 + UN3 day 3, n = 10; Saline day 7, n = 18; Comb1 + UN3 day 7, n = 24. For granulation tissue area and microvascular density measurements: Saline day 3, n = 3; Comb1 + UN3 day 3, n = 5; Saline day 7, n = 9; Comb1 + UN3 day 7, n = 10