Fig. 4

TNFα, TCP-1/TNFα, IFNγ or TCP-1/IFNγ single treatment enhanced antitumor immunity. a Immunofluorescence staining of CD8⁺ and CD4⁺ cells in the tumor mass. b Quantification of CD8⁺ and CD4⁺ cells in the tumor mass. TCP-1/TNFα and TCP-1/IFNγ single treatment significantly increased CD8⁺ and CD4⁺ cell infiltration into the tumor. c CD3⁺ cells in the spleen and peripheral blood detected by flow cytometry. TCP-1/TNFα and TCP-1/IFNγ single treatment increased CD3⁺ cells production in the spleen while decreased or didn’t change its level in peripheral blood, suggesting the treatments increased CD3⁺ cells at tumor site. Data were presented as mean ± SEM. *P < 0.05. **P < 0.01. ***P < 0.001