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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Amelioration of autoimmune arthritis by adoptive transfer of Foxp3-expressing regulatory B cells is associated with the Treg/Th17 cell balance

Fig. 4

Immunosuppressive action of Foxp3-trasnfected CD19+ B cells was dependent on cell contact. a, b Splenic CD19+ B cells from DBA/1J mice were transfected with Foxp3 or control plasmid DNA and then stimulated with 10 µg/ml LPS (a) or 10 µg/ml anti-IgM (b) for 72 h. CD4+CD25 T cells were stimulated with anti-CD3 and co-cultured with transfected B cells either directly (no chamber) or in the upper chamber of a transwell plate. Proliferation was determined after 3 days of culture by 3H-thymidine incorporation. Cultures were harvested and cpm were determined. Values are the mean ± SD from triplicate cultures. Data represent one of the two independent mice

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