Fig. 4

Immunosuppressive action of Foxp3-trasnfected CD19⁺ B cells was dependent on cell contact. a, b Splenic CD19⁺ B cells from DBA/1J mice were transfected with Foxp3 or control plasmid DNA and then stimulated with 10 µg/ml LPS (a) or 10 µg/ml anti-IgM (b) for 72 h. CD4⁺CD25⁻ T cells were stimulated with anti-CD3 and co-cultured with transfected B cells either directly (no chamber) or in the upper chamber of a transwell plate. Proliferation was determined after 3 days of culture by ³H-thymidine incorporation. Cultures were harvested and cpm were determined. Values are the mean ± SD from triplicate cultures. Data represent one of the two independent mice