Fig. 2

The frequencies of CD4⁺ and CD8⁺ Treg populations were increased in the peripheral blood of patients with LP. PBMCs obtained from patients with LP (n = 18, closed circles) and HC (n = 22, open circles) were assessed by flow cytometry for the presence of (a) CD4⁺ Tregs (CD3⁺CD4⁺CD25⁺Foxp3⁺CD127^low/−) and CD8⁺ Tregs (CD3⁺CD8⁺CD25⁺Foxp3⁺CD127^low/−), b thymus-derived CD4⁺ Tregs (CD4⁺CD45RA⁺Foxp3^low) and thymus-derived CD8⁺ Tregs (CD8⁺CD45RA⁺Foxp3^low), and (c) peripherally derived CD4⁺ Tregs (CD4⁺CD45RA⁻Foxp3^high) and peripherally derived CD8⁺ Tregs (CD8⁺CD45RA⁻FoxP3^high). The results are shown as the mean ± SEM. *P < 0.05, **P < 0.01 when compared with the HC group