Table 4 Literature review of the investigated inflammation- and fibrosis-associated genes in bronchiolitis obliterans (BO), idiopathic pulmonary fibrosis (IPF) and organizing pneumonia (OP) humans and mice
References | Pulmonary disease: no. of cases/controls | Materials | Investigated parameters | Methods |
|---|---|---|---|---|
Human | ||||
Magnan et al. [16] | BO: 6/7 | BAL (TBB) from lung transplant recipients | TGFB1, IL-6, TGFB2 | Immunoassay for cytokine determination Immunohistochemistry |
Taghavi et al. [19] | BO: 38/34 | BAL (TBB) from lung transplant patients | MMP2, MMP8, MMP9, TIMP1, TIMP2, BAL cell count | ELISA |
Ramirez et al. [22] | BO: 13/21 | BALF from lung transplant recipients | IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IFN-γ, GM-CSF, TNF-α, TGFB1, fibronectin gene expression, gelatinolytic activity | Multiplex Bead Immunoassay ELISA Gelatin zymography |
Jonigk et al. [10] | BO: 12/12 | Explanted lung allografts | BMP4, BMPR2, LOX, MMP14, PLAU, CCL5, THBS1, TIMP1, TGFB1, COL3A1, TIMP2, TGFBR2, BMP2, PLA1, EDN1, COL1A2, COL4A1 | RNA microarray (45 target genes) |
Mouse | ||||
Majeski et al. [23] | OP: | Reovirus 1/L induction of intraluminal fibrosis | Il-6, Ccl2 | RiboQuant multiprobe ribonuclease protection assay ELISA |
Phillips et al. [18] | IPF: 3–6 mice for each treatment group (bleomycin/saline) | Bleomycin mouse model | Cxcr4, Cxcl12, Col1, CD45, CD34, α-SMA, Col3 | FACS, ELISA, qRT-PCR, Immunohistochemistry |
Chen et al. [17] | BO: 4/4 | Heterotopic tracheal transplantation from TIMP-1 null (-/-) mutation and wild-type (TIMP-1 +/+) mice | Î’eta-actin, Mmp3, Mmp7, Mmp9, Mmp12, Timp1, Timp2, Timp3, Timp4 | Immunohistochemistry qRT-PCR |
Xu [24] | BO: 6/6 | Heterotopic tracheal transplantation | Cxcr4 | Immunohistochemistry Immunofluorescence Western blot |
Vittal et al. [20] | IPF: | Bleomycin mouse model | Tgfb1, FAK (PTK2), PKB/Akt | qRT-PCR Western blot Immunohistochemistry |
Shi et al. [21] | IPF: | Bleomycin mouse model | Tgfb1, Smad3 | Immunohistochemistry |