Fig. 2
From: Validation of an IFNγ/IL2 FluoroSpot assay for clinical trial monitoring
Linearity of the IFNγ/IL2 FluoroSpot assay. Magnitude of IFNγ, IL2, and IFNγ + IL2 responses of donor S25 (a) and S26 (b) within the IFNγ/IL2 FluoroSpot assay as a function of cell density. Depicted are the number of mitogen-specific IFNγ, IL2 and IFNγ + IL2 SFC/well after stimulation with 2 µg/ml PHA-L; SFC spot forming cells. R 2 = coefficient of determination