TY - JOUR AU - Körber, Nina AU - Behrends, Uta AU - Hapfelmeier, Alexander AU - Protzer, Ulrike AU - Bauer, Tanja PY - 2016 DA - 2016/06/14 TI - Validation of an IFNγ/IL2 FluoroSpot assay for clinical trial monitoring JO - Journal of Translational Medicine SP - 175 VL - 14 IS - 1 AB - The FluoroSpot assay, an advancement of the ELISpot assay, enables simultaneous measurement of different analytes secreted at a single-cell level. This allows parallel detection of several cytokines secreted by immune cells upon antigen recognition. Easier standardization, higher sensitivity and reduced labour intensity render FluoroSpot assays an interesting alternative to flow-cytometry based assays for analysis of clinical samples. While the use of immunoassays to study immunological primary and secondary endpoints becomes increasingly attractive, assays used require pre-trial validation. Here we describe the assay validation (precision, specificity and linearity) of a FluoroSpot immunological endpoint assay detecting Interferon γ (IFNγ) and Interleukin 2 (IL2) for use in clinical trial immune monitoring. SN - 1479-5876 UR - https://doi.org/10.1186/s12967-016-0932-7 DO - 10.1186/s12967-016-0932-7 ID - Körber2016 ER -