Fig. 6

Down regulation by TWEAK-binding IgGs of the in vitro endothelium CCL-2 secretion. ELISA analysis of CCL-2 levels in the supernatants of HUVECs stimulated by TWEAK (100 ng/ml) or not (medium) and co-incubated with a commercially available goat polyclonal blocking anti-TWEAK antibody (5 µg/ml) or with purified human serum IgG negative for TWEAK-binding IgG test (50 ng/ml) or with purified PsoA patient TWEAK-binding IgG (50 ng/ml). Note the downregulation of the increased secretion of CCL-2 under TWEAK exposure when cells are incubated with purified PsoA patient TWEAK-binding IgG. *p < 0.01, **p < 0.001, ***p < 0.0001 according to Student’s t test