Fig. 3

Detection of serum anti-TWEAK auto-antibodies. a Western blot assay using recombinant human TWEAK. Line 1 Positive patient with PsoA. Line 2 Negative patient with PsoA. Line 3 Negative control serum. On line 1, 2 and 3, sera are 1:50 diluted. Line 4 Positive control based on revelation with a commercially available polyclonal anti-TWEAK antibody. Line 5 Negative control based on serum omission. b Immunofluorescence test. Serum are incubated on wild-type HEK cells and on TWEAK-transfected HEK cells. Membrane and cytoplasmic fluorescence staining patterns specifically observed with an anti-TWEAK antibody positive patient serum only on the transfected wells are indicated by white arrows