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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: Resveratrol increases AdipoR1 and AdipoR2 expression in type 2 diabetic nephropathy

Fig. 7

The effect of resveratrol on the activities of AMPK–SIRT1–PGC-1α signaling, PPARα, FoxO, and apoptosis in HGECs exposed to high-glucose medium. Cultured HGECs in HG with or without resveratrol were transfected with 50 nmol/L control siRNA, 50 nmol/L AdipoR1 or AdipoR2 siRNA using transfection reagent (G-Fectin). Approximately 48 h after transfection, protein lysates (10 μg) were analyzed by Western blot for AdipoR1 and AdipoR2, phospho-Thr172 AMPK, total AMPK, SIRT1, PGC-1α, and PPARα. a Representative results for phospho-Thr172 AMPK, total AMPK, SIRT1, PPARα, PGC-1α and β-actin and quantitative analyses for SIRT1, PPARα and PGC-1α relative to β-actin and phospho-Thr172 AMPK/total AMPK in HGECs exposed to high-glucose medium without resveratrol. **p < 0.01 compared with HG + siRNA control. b Representative results for AdipoR1 and AdipoR2, phospho-Thr172 AMPK, total AMPK, SIRT1, PPARα, PGC-1α and β-actin. c Quantitative analyses for AdipoR1 and AdipoR2, SIRT1, PPARα and PGC-1α relative to β-actin and phospho-Thr172 AMPK/total AMPK d Representative results for phospho-Ser256 FoxO1 and total FoxO1, phospho-Ser253 FoxO3a and total FoxO3a and β-actin and quantitative analyses for phospho-Ser256 FoxO1/total FoxO1 and phospho-Ser253 FoxO3a/total FoxO3a. *p < 0.05 compared with HG + siRNA control. **p < 0.05 compared with HG + siRNA control and p < 0.01 compared with the other groups. e Representative immunohistochemical staining for TUNEL-positive HGECs with quantitative analysis. **p < 0.01 compared with the other groups. AdipoR adiponectin receptor, AMPK 5′-adenosine monophosphate-activated protein kinase, SIRT1 silent information regulator T1, PPARα peroxisome proliferator-activated receptorα, PGC-1α PPARγ co-activator 1α, HG high glucose, Res resveratrol, siRNA small interfering RNA, FoxO class O forkhead box

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