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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: MicroRNA-142-5p contributes to Hashimoto’s thyroiditis by targeting CLDN1

Fig. 4

miR-142-5p inhibits CLDN1 expression in vitro. a The differential gene expression by gene array comparing the primary thyrocytes upregulated miR-142-5p with lentivirus vector and control vector. b The potential gene candidates were investigated using qRT-PCR in miR-142-5p transducted thyrocytes, the level of CLDN1 expression showed most reduction after transduction. *P = 0.032. c The expression of CLDN1 mRNA measured using qRT-PCR and protein by Western blot was analyzed after infection with miR-142 expression lentivirus or negative control in thyrocytes. *P = 0.032, # P = 0.009. d Luciferase reporter constructs of wild-type and mutant target sites in the 3ʹ-UTR of CLDN1 mRNA. Red indicates the miR-142-5p binding site, which was conservative in Homo sapiens (Hsa) and Pan troglodytes (Ptr). Blue indicates mutant sequences. e The transfection of miR-142-5p mimics dramatically increased the expression of miR-142-5p but not of miR-142-3p in HEK293T. f Luciferase construct containing the miR-142-5p binding site in the CLDN1 3ʹ-UTR (wild-type or mutant) was transfected and assayed in HEK293T. NS not significant

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