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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Cathepsin A inhibition attenuates myocardial infarction-induced heart failure on the functional and proteomic levels

Fig. 6

Effect of cathepsin A inhibition in an in vitro model of ischemia. Cells were incubated for 24 h with 10 μM SAR1 or DMSO as a solvent-only control in simulated ischemia culture conditions (hypoxia and serum deprivation, see Methods section for details). a Quantitative analysis of caspase-3 activity in the total cell lysate of cells treated with SAR1 in standard conditions (normoxia; DMEM supplemented with FCS) and simulated ischemia (hypoxia; DMEM serum-free). b Cells in simulated ischemia were stained with YO-PRO-1 or PI and evaluated by flow cytometry. The percentage of cells positive to YO-PRO-1 or to PI was calculated. Results are expressed as mean ± SEM. Differences significant at p < 0.05 are marked with an asterisk (*)

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