Fig. 1

Antigen construction and expression. a Nomenclature and location of various domains of NoV VLPs. b 3D structure of VP1 encoded by ORF2 of human NoV (GII.4) (GenBank accession# AY038600.3) and of the P domain (Pd) cloned into bacterial vector system (for details see ‘‘methods’’). Numbers in (a, b) represent the amino acid numbers. c The Western blot of the bacterially expressed and purified Pd protein reacted with anti-Pd hyper-immune serum raised in mice. The recombinant Pd protein was separated by the native PAGE