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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Proteomic analysis of short-term preload-induced eccentric cardiac hypertrophy

Fig. 4

Validation of proteomics data. a Real time of mtDNA gene Cox I, Cox II, Uqcrc1 and nuclear genes Atp5a1. b Representative Western blots showing change in the expression of 60 kDa heat shock protein, mitochondrial (HSPD1), Polymerase I and transcript release factor (PTRF), ß-enolase (Eno3), COP9 signalosome complex subunit 4 (COPS4), Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3), Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MYL2), NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (Ndufs1), Heat shock cognate 71 kDa protein (HSPA8), and α-1-antitrypsin in respective experimental groups. c, d Graphs showing relative band intensity as revealed by Western blot analyses. Values are mean ± SEM; n = 4–5/group; analysis per heart in duplicate; *P < 0.05 vs. sham

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