Fig. 5
IL-17A-induced resistance to daunorubicin is dependent on Akt activation. Nalm-6 cells (a, b) and patient B-ALL cells (c, d) isolated from 4 B-ALL patients were pre-treated with the PI3K/Akt inhibitor LY294002 at 10 μM or perifosine at 2 μM for 2 h and treated with or without IL-17A at 50 ng/ml for 24 h. Then, the cells were further treated with daunorubicin at 200 nM for 24 h. The cell viability and apoptosis were determined using CCK-8 assay and Annexin V staining, respectively. Statistical data representing at least three independent experiments were shown