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Table 4 MSC thawing: pre and post-thaw MSC immunosuppressive properties

From: Clinical-scale expansion of mesenchymal stromal cells: a large banking experience

 

(%)

Min.

Max.

Mean ± SD

Day 0

Recovery

61

84

72.8 ± 10.4

Viability

61

71

65.1 ± 4.4

Immunosuppression

5

5

5.0 ± 0.0

Day 1

Recovery

21

54

41.8 ± 12.9

Viability

86

91

88.8 ± 2.1

Immunosuppression

0

10

2.9 ± 4.1

Day 2

Recovery

19

40

30.3 ± 11.3

Viability

78

87

83.9 ± 4.0

Immunosuppression

4

19

10.3 ± 7.4

Day 3

Recovery

21

36

26.8 ± 10.8

Viability

74

97

85.5 ± 16.3

Immunosuppression

34

47

40.5 ± 8.6

Day 4

Recovery

21

34

28.3 ± 6.5

Viability

89

93

90.3 ± 2.3

Immunosuppression

40

43

41.8 ± 2.2

Day 5

Recovery

31

42

36.3 ± 7.5

Viability

85

91

88.0 ± 4.2

Immunosuppression

42

44

42.6 ± 1.3

  1. Results are shown for MSC recovery, viability and immunosuppressive properties (%) at different times after thawing and replating in T-flasks with complete media