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Table 2 Marker associations according to central HER2 status

From: MYC copy gain, chromosomal instability and PI3K activation as potential markers of unfavourable outcome in trastuzumab-treated patients with metastatic breast cancer

 

HER2-negative

HER2-positive

p value

Ki67 (%)

 Median (range)

38 (1–90)

40 (1–90)

0.12

HER2 copies (FISH)

 Median (range)

2 (1–9)

13 (2–39)

<0.001

 Mean (±SD)

3 (1)

14 (8)

 

HER2 ratio (FISH)

 Median (range)

1 (1–8)

5 (1–28)

<0.001

 Mean (±SD)

1 (1)

7 (4)

 

CEN17

 Median (range)

2 (1–4)

2 (1–4)

0.006

 Mean (±SD)

2 (1)

2 (1)

 

CEN8

 Median (range)

3 (1–9)

2 (1–5)

0.80

 Mean (±SD)

3 (1)

3 (1)

 

CEN7

 Median (range)

2 (1–4)

2 (1–5)

0.50

 Mean (±SD)

2 (1)

2 (1)

 
 

N

%

N

%

p value

IHC, categorical

 ER (N = 229)

  Negative

16

17.8

60

43.2

<0.001

  Positive

74

82.2

79

56.8

 

 PgR (N = 229)

  Negative

32

35.6

89

64

<0.001

  Positive

58

64.4

50

36

 

 mTOR (N = 229)

  Negative

36

40

48

34.5

0.40

  Positive

54

60

91

65.5

 

 PTEN (N = 183)

  Negative (0–1)

47

65.3

55

49.5

0.036

  Positive (2–3)

25

34.7

56

50.5

 

 c-MYC (N = 188)

  Both negative

19

24.4

41

37.3

0.011

  Both positive

27

34.6

31

28.2

 

  Cytoplasmic only

28

35.9

22

20

 

  Nuclear only

4

5.1

16

14.5

 

Mutations

 PIK3CA (N = 176)

  WT

49

69

88

83.3

0.020

  Mutant

22

31

17

16.2

 

Gene CN by qPCR

 MET (N = 159)

  No gain

42

71.2

77

77

0.41

  Gain

17

28.8

23

23

 

 MYC (N = 159)

  No gain

54

90

90

90.9

0.85

  Gain

6

10

9

9.1

 

Gene status by FISH

 MET (N = 164)

  Non-amplified

56

100

104

96.3

0.15

  Amplified

  

4

3.7

 

 MYC (N = 173)

  Non-amplified

56

88.9

86

78.2

0.077

  Amplified

7

11.1

24

21.8

 

 TOP2A (N = 226)

  Non-amplified

82

93.2

74

53.6

<0.001

  Amplified

6

6.8

64

46.4

 

 CEN17 (N = 226)

  Normal

82

93.2

119

86.2

0.10

  Increased

6

6.8

19

13.8

 

 CEN7 (N = 170)

  Normal

54

90

101

91.8

0.69

  Increased

6

10

9

8.2

 

 CEN8 (N = 174)

  Normal

47

74.6

79

71.2

0.63

  Increased

16

25.4

32

28.8

 

 CEN profiles (N = 170)

  0 chromosomes

40

66.7

60

54.5

0.21

  1 chromosome

13

21.7

41

37.3

 

  2 chromosomes

6

10

8

7.3

 

  3 chromosomes

1

1.7

1

0.9

 

mRNA

 MET (N = 139)

  Low

36

63.2

36

43.9

0.025

  High (≥50th perc.)

21

36.8

46

56.1

 

 MYC (N = 140)

  Low

36

63.2

37

44.6

0.031

  High (≥50th perc.)

21

36.8

46

55.4

 
  1. Significant p values are shown in italics