Fig. 2

Lenalidomide enhances pericyte coverage of residual mCRC vessels. a Lenalidomide significantly enhanced mature pericyte coverage, reported as mean fluorescence intensity (MFI) of α-SMA (p = 0.04) and NG-2 (P = 0.0002) co-localized on vessel areas (MECA32), the results were compared with one-way ANOVA. The values are presented as mean ± SEM of MOF, 3 mice with mCRC tumorgraft per group were included in the experiments. Post analysis comparisons of each group was performed with Bonferroni post-test analysis, resulting in significant p values of lenalidomide against vehicle (*p < 0.05, for α-SMA) and against 5FU (***p < 0.001, for NG2) b Representative immunofluorescence picture showing co-localization (merge) of NG2 pericyte marker (green) and Meca32 endothelial marker (red) in tumor samples from mice treated with lenalidomide and control vehicle. MOF, Microscopic observation fields; (*), P ≤ 0.05; (**), P ≤ 0.01; (***), P ≤ 0.001