Fig. 6

Prdx1 induce IL-6 and TNF-α production in INF-γ primed macrophage. Human recombinant Prdx1 (rPrdx1) at 11 μg/mL (a) or LPS (50 pg/mL) (b), that was either pretreated with PMB (10 μg/mL) or not, was added to human monocyte derived dendritic cells for 24 h before the culture supernatants were collected and analyzed for IL-6 levels. INF-γ (20 U/mL) pretreated human monocyte derived macrophages (c) or mouse macrophage RAW264.7 cells [12] were incubated with Prdx1 (11 μg/mL) or LPS (50 pg/mL) for 24 h before the supernatants were harvested and analyzed for IL-6 (c) or TNF-α [12] levels. Solvent vehicles diluted in the culture media were used as the negative controls. Data represented means of triplicate samples; error bars represented SEMs. *P < 0.05, and ***P < 0.001