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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: GIFT4 fusokine converts leukemic B cells into immune helper cells

Fig. 4

GIFT4 treatment induced the expansion of autologous T cells. PBMC were isolated from the peripheral blood of CLL patients, and stimulated with GIFT4, GM-CSF and IL-4 or PBS for 5 days. T cells in the PBMC before treatment (a), or after 5-day culture with GIFT4, GM-CSF and IL-4 or PBS (b) were profiled by FACS with anti-CD3 antibody; and T cell percentage and the absolute T cell number fold change were calculated from three independent experiments using samples from subjects No. 7, 10, and 11 (c, d). e CSFE-labeled autologous T cells were co-cultured with GIFT4-CLL cells (Dark), GM-CSF and IL-4 treated CLL cells (Gray), untreated CLL cells (White), or with GIFT4 protein (Dash) for 4 days. f Alternatively, anti-human CD80 (Gray), CD86 (White) neutralizing antibodies or isotype control (Black) were pre-incubated with GIFT4-CLL cells before co-cultured with CFSE-labeled autologous T cells. T cell division was analyzed by FACS (e, f)

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