Fig. 5

Tracking of injected MIC_Cop cells in mice. SPCs from naïve SJL/J mice were loaded with Copaxone^® (Cop) o/n and then treated with 50 µg/mL MMC (MIC_Cop). 2 × 10⁷ MIC_Cop were labeled with 20 MBq ¹¹¹Indium (In)-oxine and injected into the tail vein of the recipient mouse. Twenty-four hours later the animals were euthanized, thoroughly perfused with Ringer’s solution and single organs were harvested and weighed. The activity of tissue samples as well as of an aliquot of the administered labeled cell suspension was determined for each animal in a γ-counter and calculated as  % of total injected dose (ID) per gram tissue. Shown is the in vivo distribution of activity in the indicated organs of four mice depicted as mean ± SD (LN lymph node)