Fig. 3
Cytokine expression of peripheral lymphocytes from MICCop-treated EAE mice after in vitro-restimulation with PLP and Copaxone®. Splenocytes (SPCs; left column) and lymph node cells (LNCs; right column) were isolated from PBS- (control, black, n = 9) and MICCop-treated (MICCop, grey, n = 8) EAE mice and restimulated in vitro with 20 µg/mL PLP139-151 or Copaxone® (Cop). After 72 and 96 h, supernatants were collected and concentrations of TGF-β (a and b) and IL-10 (c and d) were determined by ELISA. Unstimulated cells represent basic cytokine expression. The mean ± standard error of the mean (SEM) was calculated for values measured in duplicate. Treatment groups were compared using unpaired Student’s t test (***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant)