Fig. 2
MICCop therapy suppresses the PLP-specific T-cell response. SJL/J mice were induced to exhibit clinical signs of EAE. In their first phase of remission, on days 21–23 post disease induction, mice were treated with MICCop (white triangles) or PBS (control, black squares). LNCs (a) and SPCs (b) of MICCop-treated (n = 8) and control (n = 9) EAE mice were harvested on day 49 after disease induction. Cells of each treatment group were restimulated in vitro with PLP139–151 for 48 h at the indicated concentrations (abscissa). Proliferation was determined by [3H]-thymidine incorporation and is displayed as x-fold increase in relation to unstimulated cells (ordinate). The mean ± standard error of the mean (SEM) of a sixtuplicate setup was calculated. One representative of three independent experiments is shown. Unpaired Student’s t test was performed for comparison of the treatment groups (*p < 0.05; **p < 0.01; ***p < 0.001)