Fig. 2

MEK1/2 and p38 measurement in CD56^brightCD16^dim/− NK cells. For a representative individual, CD56^brightCD16^dim/− NK cells were identified using CD56 and CD16 (a). CD56^brightCD16^dim/− NK cell MEK1/2 was determined on a histogram (b) and comparisons were drawn between CFS/ME and the NFC under different stimulatory conditions (c). In comparison to the NFC, MEK1/2 was significantly increased in CFS/ME patients after K562 cell incubation (*p < 0.05). p38 MFI in CD56^brightCD16^dim/− NK cells was also measured on histograms (d) and comparisons were drawn against the CFS/ME patients and the NFC (e) before and after stimulation. In CFS/ME patients, p38 was significantly increased (*p < 0.05) after K562 incubation compared to the NFC. Data are presented as MFI with interquartile range